It is widely used in synthetic biology and metabolic engineering for driving. Our aim was to search for promoters for the expression of pediocin (pedb) and nisin (nisi) immunity genes, which could allow the selection of a wide variety of transformed lactic acid bacteria (lab) and. The p32 promoter is a strong, constitutive promoter derived from the lactococcus lactis bacteriophage 'bil309'.
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In this study, eight different putative strong constitutive promoters were identified through transcriptional analysis of l. It is widely used in synthetic biology and metabolic engineering for driving. To test if our lactococcus lactis constitutive promoters function well within e.
Lactis n8 and were investigated for their capability to drive nisz gene expression with.
We evaluated five different promoters in two different. The promoters of 32 genes constitutively expressed (table s3) under both culture conditions were cloned from the l. Lactis n8 and were investigated for their capability to drive nisz gene. This plasmid can be propagated in a broad host range, including lactococcus lactis, escherichia coli, bacillus subtilis and several lactobacillus strains.
Zeocin® is an invivogen trademark. In this study, eight different putative strong constitutive promoters were identified through transcriptional analysis of l. The p32 promoter is a strong, constitutive promoter derived from the lactococcus lactis bacteriophage 'bil309'.
